Non-Mammalian enzymes:
- Trypzean (Sigma) : recombinant, maize-derived trypsin- TrypLE (Invitrogen) : recombinant microbial
- Accutase and Accumax (Innovative Cell Technologies)
Criteria for Subculture Cells in Monolayer
- Density of culture- Exhaustion of medium
- Time since last subculture
- Requirements for other procedures
- cells should not be subculture while still within the LAG phase.
- between the middle of log phase and the time before entering the plateau phase.
Replacement of Medium
- A drop in pH- [Cell] : Cultures at a high [cell] exhaust the medium faster than those at lower [ ]
- Cell type
- Morphological deterioration
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